pcdna3 plk1 829  (Addgene inc)

Journal: American Journal of Respiratory Cell and Molecular Biology

Article Title: Plk1 Regulates Caspase-9 Phosphorylation at Ser-196 and Apoptosis of Human Airway Smooth Muscle Cells

doi: 10.1165/rcmb.2021-0192oc

Figure Lengend Snippet: Figure 3. Time course of Plk1 expression and Casp9 phosphorylation at Ser-196 in response to stimulation with PDGF and epidermal growth factor. (A and B) HASM cells were treated with 10 ng/ml PDGF (A) or epidermal growth factor (B) for different time points. Protein phosphorylation and expression were evaluated by IB. Plk1 expression and Casp9 phosphorylation at Ser-196 are time dependent. Data are mean values of four to six nonasthmatic cell cultures from three donors. Error bars indicate SD. *P , 0.05 and **P = 0.01. One-way ANOVA was used for statistical analysis.

Article Snippet: Plk1 cDNAwas cut off from pcDNA3 33 Flag-Plk1 (5) and subcloned to pLentipuro (Addgene #39481) at XhoI and ApaI sites.

Techniques: Expressing, Phospho-proteomics

Journal: Scientific reports

Article Title: MicroRNA miR-509 Regulates ERK1/2, the Vimentin Network, and Focal Adhesions by Targeting Plk1.

doi: 10.1038/s41598-018-30895-8

Figure Lengend Snippet: Figure 1. Plk1 is regulated by miR-509 at mRNA and protein levels in smooth muscle cells. (A) Human airway smooth muscle (HASM) cells were transfected with either 20 nM miR-control (miR-Ctrl) or miR-100, or they were untransfected, for 3 days. Blots of the HASM cells were probed with antibodies against Plk1 and glyceraldehyde-3-phosphate dehydrogenase (GAPDH). Data are mean ± SD (n = 4). NS, not significant. (B) All three online miR search tools predict 3′UTR of human Plk1 as a target of miR-509. (C) Sequence alignment between miR-509 and 3′UTR of human Plk1. (D) Blots of untransfected HASM cells and cells transfected with either miR-Ctrl or miR-509 for 3 days were probed with antibodies against Plk1 and GAPDH. Data are mean ± SD (n = 4, **p < 0.01). (E) HASM cells were transfected with either 20 nM miR-Ctrl or miR-509, or they were untransfected for 3 days. Plk1 mRNA in the cells was assessed by RT-qPCR (reverse transcription quantitative real-time polymerase chain reaction). Data are mean ± SD (n = 4, **p < 0.01). (F) HASM cells were untransfected, or transfected with either 20 nM control RNA or miR-509 inhibitor for 2 days. miR-509 inhibitor is small, chemically modified single-strand RNA molecules designed to specifically bind to and inhibit endogenous miR-509. Blots of the cells were probed with antibodies against Plk1 and GAPDH. Data are mean ± SD (n = 4, **p < 0.01). (G) mRNA of Plk1 in untransfected cells and cells transfected with either 20 nM

Article Snippet: Plk1 cDNA was cut off from pcDNA3 3xFlag-Plk17 and subcloned to pLenti-puro (Addgene #39481) at XhoI and ApaI sites. pLenti-puro has 3′UTR and poly A of BGH, which does not have miR-509 targeting sequence, and is resistant to miR-509.

Techniques: Transfection, Control, Sequencing, Quantitative RT-PCR, Reverse Transcription, Real-time Polymerase Chain Reaction, Modification

Journal: Scientific reports

Article Title: MicroRNA miR-509 Regulates ERK1/2, the Vimentin Network, and Focal Adhesions by Targeting Plk1.

doi: 10.1038/s41598-018-30895-8

Figure Lengend Snippet: Figure 2. Asthmatic human airway smooth muscle cells display reduced miR-509 expression, increased Plk1 expression, and enhanced proliferation and migration. (A) miR-509 expression in control and asthmatic HASM cells were determined using the experimental procedure described in Materials and Methods. miR-509 expression is reduced in asthmatic smooth muscle cells (Control cells from 5 donors, asthmatic cells from 4 donors, *p < 0.05). However, expression of Plk1 at mRNA (B) and protein (C) levels is higher in asthmatic HASM cells (Control cells from 5 donors, asthmatic cells from 4 donors, *p < 0.05). (D) Control and asthmatic HASM cells were treated with 10 ng/ml PDGF for 3 days followed by cell counting. The proliferation of asthmatic HASM cells is increased as compared to control cells (Control cells from 5 donors, asthmatic cells from 4 donors, *p < 0.05). (E–H). Control and asthmatic HASM cells were replated in 6-well dishes for 5 hours, and migration of these cells was then evaluated by time-lapse microscopy for additional 16 h. NIH ImageJ software was used to analyze cell migration speed, directionality, accumulated distance, and Euclidean distance (*p < 0.05; ***p < 0.01; control cells, n = 58 from 5 donors; asthmatic cells, n = 72 from 4 donors). Student’s t-test was used for statistical analysis.

Article Snippet: Plk1 cDNA was cut off from pcDNA3 3xFlag-Plk17 and subcloned to pLenti-puro (Addgene #39481) at XhoI and ApaI sites. pLenti-puro has 3′UTR and poly A of BGH, which does not have miR-509 targeting sequence, and is resistant to miR-509.

Techniques: Expressing, Migration, Control, Cell Counting, Time-lapse Microscopy, Software

Journal: Scientific reports

Article Title: MicroRNA miR-509 Regulates ERK1/2, the Vimentin Network, and Focal Adhesions by Targeting Plk1.

doi: 10.1038/s41598-018-30895-8

Figure Lengend Snippet: Figure 3. Role of miR-509 in cell proliferation, c-Abl expression, MEK1/2 and ERK1/2 phosphorylation. (A) HASM cells were transfected with miR-Ctrl or miR-509. One day after transfection, they were stimulated with 10 ng/ml PDGF for additional 1–3 days followed by assessment of cell numbers. Exposure to miR-509 inhibits the PDGF-induced proliferation. Data are mean ± SD (n = 5, **p < 0.01). (B) Treatment with miR-509 does not affect c-Abl protein expression. Blots of HASM cells transfected with either miR-Ctrl or miR-509 for 3 days were probed with antibodies against c-Abl and GAPDH. Data are mean ± SD (n = 5). NS, not significant. (C) HASM cells treated with miR-Ctrl or miR-509 for 3 days, or stable Plk1 (miR-509 resistant) expressing cells were treated with miR-509 for 3 days (rescue cells). Cell extracts were evaluated by immunoblot analysis. Data are mean ± SD (n = 5, **p < 0.01). (D) Representative immunoblots illustrating the role of Plk1 in phosphorylation of MEK1/2 (MEK1: Ser-218/Ser-222; MEK2: Ser-222/Ser-226) and ERK1/2 (ERK1: Thr-202/ Tyr-204; ERK2: Thr-185/Tyr-187). HASM cells treated with miR-Ctrl or miR-509, or rescue cells (see above) were stimulated with 10 ng/ml PDGF for 10 min or left unstimulated. MEK1/2 and ERK1/2 phosphorylation in these cells was evaluated by immunoblot analysis. Rescue of Plk1 in miR-509 treated cells restores the PDGF-

Article Snippet: Plk1 cDNA was cut off from pcDNA3 3xFlag-Plk17 and subcloned to pLenti-puro (Addgene #39481) at XhoI and ApaI sites. pLenti-puro has 3′UTR and poly A of BGH, which does not have miR-509 targeting sequence, and is resistant to miR-509.

Techniques: Expressing, Phospho-proteomics, Transfection, Western Blot

Journal: Scientific reports

Article Title: MicroRNA miR-509 Regulates ERK1/2, the Vimentin Network, and Focal Adhesions by Targeting Plk1.

doi: 10.1038/s41598-018-30895-8

Figure Lengend Snippet: Figure 4. Vimentin network organization and focal adhesion size are regulated by miR-509. (A) HASM cells treated with miR-control or miR-509, and rescue cells were stained for vimentin and paxillin. Cell images were taken using a Zeiss LSM880 microscope with Airyscan. The images of vimentin filaments and paxillin staining (>1 µM in length) in cell protrusions were used for Imaris quantitative analysis. White scale bar = 10 μm, yellow scale bar = 3 μm. Imaris software was utilized to 3D-render vimentin filaments and paxillin surfaces. 3D-rendered vimentin is green, paxillin contacting vimentin is cyan, and paxillin alone is red. (B) Distance transformation was utilized to quantify the percent of vimentin filaments contacting paxillin surfaces using Imaris software. (C–E) The length of vimentin filaments, paxillin surfaces and paxillin area were quantified using Imaris software. Statistics used in all graphs were one-way ANOVA with Tukey’s post-hoc test, n = 5, *p < 0.05. (F) Representative immunoblots showing the effects of miR-509 and Plk1 on vimentin Ser-56 phosphorylation. Extracts of HASM cells treated with miR-Ctrl, miR-509 or miR-509 plus Plk1 expression construct were immunoblotted with antibodies against phospho-vimentin (Ser-56), total vimentin, and GAPDH. (G) Vimentin phosphorylation is normalized to the level obtained from cells treated with miR-Ctrl. Data are mean ± SD (n = 4, **p < 0.01). One-way ANOVA was used for statistical analysis of B–E and G.

Article Snippet: Plk1 cDNA was cut off from pcDNA3 3xFlag-Plk17 and subcloned to pLenti-puro (Addgene #39481) at XhoI and ApaI sites. pLenti-puro has 3′UTR and poly A of BGH, which does not have miR-509 targeting sequence, and is resistant to miR-509.

Techniques: Control, Staining, Microscopy, Software, Transformation Assay, Western Blot, Phospho-proteomics, Expressing, Construct

Journal: Scientific reports

Article Title: MicroRNA miR-509 Regulates ERK1/2, the Vimentin Network, and Focal Adhesions by Targeting Plk1.

doi: 10.1038/s41598-018-30895-8

Figure Lengend Snippet: Figure 5. miR-509 negatively regulates smooth muscle cell migration through Plk1. (A) Migratory paths of individual cells treated with miR-Ctrl or miR-509, or rescue cells. Treatment with miR-509 reduces accumulated distance (B), Euclidean distance (C), speed (D) and directionality (E), which is restored in rescue cells. Data are mean values of 31 miR-Ctrl treated cells, 36 miR-509 treated cells and 23 rescue cells. Error bars indicate SD (*p < 0.05). One-way ANOVA was used for statistical analysis of B–E.

Article Snippet: Plk1 cDNA was cut off from pcDNA3 3xFlag-Plk17 and subcloned to pLenti-puro (Addgene #39481) at XhoI and ApaI sites. pLenti-puro has 3′UTR and poly A of BGH, which does not have miR-509 targeting sequence, and is resistant to miR-509.

Techniques: Migration

Journal: Scientific reports

Article Title: MicroRNA miR-509 Regulates ERK1/2, the Vimentin Network, and Focal Adhesions by Targeting Plk1.

doi: 10.1038/s41598-018-30895-8

Figure Lengend Snippet: Figure 6. Proposed model: miR-509 targets 3′UTR of Plk1 and reduces its expression, which subsequently inhibits the growth factor-induced phosphorylation of MEK1/2 and ERK1/2, and proliferation in smooth muscle cells. Reduced Plk1 expression also diminishes vimentin phosphorylation and organization of the vimentin network, focal adhesion assembly, and migration.

Article Snippet: Plk1 cDNA was cut off from pcDNA3 3xFlag-Plk17 and subcloned to pLenti-puro (Addgene #39481) at XhoI and ApaI sites. pLenti-puro has 3′UTR and poly A of BGH, which does not have miR-509 targeting sequence, and is resistant to miR-509.

Techniques: Expressing, Phospho-proteomics, Migration